amplified restriction fragment
- amplified restriction fragment
Амплифицированный рестрикционный фрагмент, АРФ — фрагмент рестрикции, который специфически модифицирован путем присоединения адапторов к его концам с целью его амплификации методом ПЦР с праймерами, комплементарными адапторам.
Англо-русский толковый словарь генетических терминов. — М.: Изд-во ВНИРО.
Арефьев В.А., Лисовенко Л.А., науч. ред. Л.И.Патрушев.
1995.
Смотреть что такое "amplified restriction fragment" в других словарях:
Restriction fragment length polymorphism — A Restriction fragment length polymorphism (or RFLP, often pronounced as rif lip ) is a variation in the DNA sequence of a genome that can be detected by breaking the DNA into pieces with restriction enzymes and analyzing the size of the… … Wikipedia
restriction fragment length polymorphism — (= RFLP) Technique, also known as DNA fingerprinting, that allows familial relationships to be established by comparing the characteristic polymorphic patterns that are obtained when certain regions of genomic DNA are amplified (typically by PCR) … Dictionary of molecular biology
Terminal restriction fragment length polymorphism — (TRFLP or sometimes T RFLP) is a molecular biology technique for profiling of microbial communities based on the position of a restriction site closest to a labeled end of an amplified gene. The method is based on digesting a mixture of PCR… … Wikipedia
amplified fragment length polymorphism — (AMP/FLP) Polymerase chain reaction amplified restriction fragment lengths consisting of variable number of tandem repeats … Forensic science glossary
Amplified fragment length polymorphism — PCR (or AFLP PCR or just AFLP) is a PCR based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990’s by [http://www.keygene.com Keygene] , AFLP uses restriction enzymes to cut … Wikipedia
Amplified Ribosomal DNA Restriction Analysis — Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (Restriction Fragment Length Polymorphism) to the gene encoding the small (16s) ribosomal subunit of bacteria. The technique involves an enzymatic… … Wikipedia
Restriction digest — A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation (this term is used for other procedures as well). Hartl and Jones describe it this way: This… … Wikipedia
amplified fragment length polymorphism — Abbreviation: AFLP A type of DNA marker, generated by the PCR amplification of restriction endonuclease treated DNA. A small proportion of all restriction fragments is amplified in any one reaction, so that AFLP profiles can be analysed by gel… … Glossary of Biotechnology
Cleaved amplified polymorphic sequence — The cleaved amplified polymorphic sequence or CAPS method is a technique in molecular biology for the analysis of genetic markers. It is an extension to the Restriction Fragment Length Polymorphism (RFLP) method, using polymerase chain reaction… … Wikipedia
Oligomer restriction — (abbreviated OR) is a procedure to detect an altered DNA sequence in a genome. A labeled oligonucleotide probe is hybridized to a target DNA, and then treated with a restriction enzyme. If the probe exactly matches the target, the restriction… … Wikipedia
cleaved amplified polymorphic sequence — A segment of DNA that can be amplified by polymerase chain reaction (PCR) and which contains a DNA sequence polymorphism. Following PCR amplification of a locus, the amplicon is treated with a restriction endonuclease. If the recognition site for … Glossary of Biotechnology